| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanylate cyclase soluble subunit alpha-1; GCS-alpha-1; Guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; Soluble guanylate cyclase large subunit; GUCY1A1; GUC1A3; GUCSA3; GUCY1A3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GCYA3 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25G96 is a rabbit monoclonal antibody that recognizes guanylate cyclase soluble subunit alpha-1 (gene GUCY1A1) in human samples and is validated for Western blot. The predicted molecular weight is 77.5 kDa, and the supplier reports an observed band at about 73 kDa.
Soluble guanylate cyclase alpha-1 (GUCY1A1) is the alpha subunit of soluble guanylate cyclase (sGC), the main intracellular receptor for nitric oxide (NO). Paired with a beta subunit that carries the NO-binding heme, it converts GTP into the second messenger cGMP. The sGC–cGMP pathway controls vascular smooth muscle relaxation and platelet function and is studied in cardiovascular research.
| Target | Guanylate cyclase soluble subunit alpha-1 (gene GUCY1A1; UniProt Q02108, human) |
|---|---|
| Clone | 25G96 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.5 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GCYA3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places guanylate cyclase soluble subunit alpha-1 in the cytoplasm. Tissue expression noted by UniProt (human): Detected in brain cortex and lung (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
