| Field | Specification |
|---|---|
| Target | |
| Alternative names | Growth/differentiation factor 15; GDF-15; Macrophage inhibitory cytokine 1; MIC-1; Gdf15; Mic1; Sbf |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Gdf15 recombinant protein (Position: S189-A302). Mouse Gdf15 shares 69.1% and 96.5% amino acid (aa) sequence identity with human and rat Gdf15, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets growth/differentiation factor 15 (gene Gdf15) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
GDF15 is a distant TGF-β superfamily member that is released in response to cellular stress, inflammation and mitochondrial dysfunction. It acts on the receptor GFRAL in the brainstem to reduce food intake and cause nausea. Circulating GDF15 rises in many conditions, and it is studied in metabolism, cachexia, pregnancy and cardiovascular disease.
| Target | Growth/differentiation factor 15 (gene Gdf15; UniProt Q9Z0J7, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Flow cytometry | Not specified by the supplier |
Immunogen
Recombinant mouse Gdf15 fragment (Ser189–Ala302), expressed in E. coli. Sequence identity with the human and rat orthologs: 69.1% and 96.5%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes growth/differentiation factor 15 as a secreted protein. Tissue expression noted by UniProt (mouse): Detected in plasma (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
