| Field | Specification |
|---|---|
| Target | |
| Alternative names | Growth/differentiation factor 15; GDF-15; Macrophage inhibitory cytokine 1; MIC-1; NSAID-activated gene 1 protein; NAG-1; NSAID-regulated gene 1 protein; NRG-1; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor; GDF15; MIC1; PDF; PLAB; PTGFB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GDF15 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20G21 is a rabbit monoclonal antibody that recognizes growth/differentiation factor 15 (gene GDF15) in human samples and is validated for Western blot. The predicted molecular weight is 34.1 kDa, and the supplier reports an observed band at about 35 kDa.
GDF15 is a distant TGF-β superfamily member that is released in response to cellular stress, inflammation and mitochondrial dysfunction. It acts on the receptor GFRAL in the brainstem to reduce food intake and cause nausea. Circulating GDF15 rises in many conditions, and it is studied in metabolism, cachexia, pregnancy and cardiovascular disease.
| Target | Growth/differentiation factor 15 (gene GDF15; UniProt Q99988, human) |
|---|---|
| Clone | 20G21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.1 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GDF15.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes growth/differentiation factor 15 as a secreted protein. Tissue expression noted by UniProt (human): Detected in plasma (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dai Chaochao et al. (2024) Identification of a distinct cluster of GDF15high macrophages induced by in vitro differentiation exhibiting anti-inflammatory activities. Frontiers in Immunology. 10.3389/fimmu.2024.1309739
