| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gelsolin; AGEL; Actin-depolymerizing factor; ADF; Brevin; GSN |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Gelsolin. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEHE-7 is a rabbit monoclonal antibody that recognizes gelsolin (gene GSN) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 85.7 kDa, and the supplier reports an observed band at about 86 kDa.
Gelsolin (GSN) is a Ca2+-regulated actin-binding protein that severs actin filaments and caps their barbed ends, and it can also nucleate new filaments. It exists as an intracellular form that remodels the cytoskeleton during cell movement and as a secreted plasma form that helps clear actin released from damaged cells. Gelsolin is studied in cell motility, cytoskeletal dynamics and familial amyloidosis.
| Target | Gelsolin (gene GSN; UniProt P06396, human) |
|---|---|
| Clone | AEHE-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 85.7 kDa |
| Observed MW | 86 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human gelsolin.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
