| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gem-associated protein 2; Gemin-2; Component of gems 2; Survival of motor neuron protein-interacting protein 1; SMN-interacting protein 1; GEMIN2; SIP1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Gemin 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25G72 is a rabbit monoclonal antibody that recognizes gem-associated protein 2 (gene GEMIN2) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 32 kDa.
Gemin2 is a core component of the SMN complex, which assembles the Sm protein ring onto small nuclear RNAs to form spliceosomal snRNPs. Gemin2 binds SMN and holds a set of Sm proteins in the correct arrangement for loading onto the RNA. It is studied in RNA processing and spinal muscular atrophy research.
| Target | Gem-associated protein 2 (gene GEMIN2; UniProt O14893, human) |
|---|---|
| Clone | 25G72 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.6 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human gemin 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places gem-associated protein 2 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
