| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pygopus homolog 2;PYGO2;PP7910; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Gemin 3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-DDX20 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24D25; isotype IgG; reactivity: Human,Mouse. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Gemin 3 Rabbit Monoclonal Antibody catalog # M05291. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: DDX20 (Pygopus homolog 2).
- Antibody format: Monoclonal; clone 24D25; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
DDX20 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Involved in signal transduction through the Wnt pathway. Reported cellular localization context: Nucleus . Tissue expression notes (as provided): Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Research relevance and current trends
- Research context keywords from the source record include: Domain Families,Epigenetics and Nuclear Signaling,Nuclear,Signaling Pathways,Stem Cells,Transcription,Zinc Finger.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate DDX20 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DDX20 expression by Western blot in cell or tissue lysates, Detect DDX20 in FFPE tissue sections by immunohistochemistry, Localize DDX20 by immunofluorescence/immunocytochemistry in cultured cells, Quantify DDX20-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 100 kDa; calculated MW: 41244 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 100 kDa
- Cellular localization (provided): Nucleus .
- Tissue details (provided): Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
