| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glial fibrillary acidic protein;GFAP;GFAP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GFAP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ECO-7 is a rabbit monoclonal antibody that recognizes glial fibrillary acidic protein (gene GFAP) in human and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 49.9 kDa, and the supplier reports an observed band at about 50 kDa.
Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein expressed mainly in astrocytes. It is the classic marker for astrocytes and for reactive astrogliosis after injury or disease. Mutations in GFAP cause Alexander disease, and GFAP levels are studied as a marker of brain injury.
| Target | Glial fibrillary acidic protein (gene GFAP; UniProt P14136, human) |
|---|---|
| Clone | ECO-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 49.9 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human glioma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): SNB19 cells.
Immunogen
Synthetic peptide based on the sequence of human GFAP.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places glial fibrillary acidic protein in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in cells lacking fibronectin. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lu Liu et al. (2017) Increased expression of Myc‑interacting zinc finger protein 1 in APP/PS1 mice. Experimental and Therapeutic Medicine. 10.3892/etm.2017.5289
