| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-interferon-inducible lysosomal thiol reductase; Gamma-interferon-inducible protein IP-30; Lysosomal thiol reductase IP30; Ifi30; Gilt; Ip30 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse GILT/IFI30, which shares 84.2% and 89.5% amino acid (aa) sequence identity with human and rat GILT/IFI30, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets gamma-interferon-inducible lysosomal thiol reductase (gene Ifi30) in mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 27.8 kDa, and the supplier reports an observed band at about 28 kDa.
IFI30 (also called GILT) is a lysosomal thiol reductase that is expressed constitutively in antigen-presenting cells and induced by interferon-γ. It reduces disulfide bonds in endocytosed proteins, helping them unfold for efficient processing and presentation on MHC class II and, in some cases, MHC class I molecules.
| Target | Gamma-interferon-inducible lysosomal thiol reductase (gene Ifi30; UniProt Q9ESY9, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 27.8 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lymphaden tissue, mouse thymus tissue, rat lymphaden tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): mouse lymphaden tissue, rat thymus tissue.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Synthetic peptide from the C-terminal region of mouse GILT/IFI30. Sequence identity with the human and rat orthologs: 84.2% and 89.5%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes gamma-interferon-inducible lysosomal thiol reductase as secreted and also located in the lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
