| Field | Specification |
|---|---|
| Target | |
| Alternative names | GTPase IMAP family member 4; Immunity-associated nucleotide 1 protein; IAN-1; hIAN1; Immunity-associated protein 4; GIMAP4; IAN1; IMAP4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GIMAP4 recombinant protein (Position: R53-R224). Human GIMAP4 shares 66.7% and 65.7% amino acid (aa) sequence identity with mouse and rat GIMAP4, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects GTPase IMAP family member 4 (gene GIMAP4) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
GIMAP4 is a member of the GIMAP family of immune-associated GTPases and binds GDP more tightly than GTP. It is expressed in lymphocytes and is thought to take part in regulating apoptosis during T cell development in the thymus.
| Target | GTPase IMAP family member 4 (gene GIMAP4; UniProt Q9NUV9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human GIMAP4 fragment (Arg53–Arg224), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 66.7% and 65.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places GTPase IMAP family member 4 in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in spleen and peripheral blood leukocytes that contain mostly T- and B-lymphocytes.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
