| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA replication complex GINS protein PSF3; GINS complex subunit 3; GINS3; PSF3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GINS3 recombinant protein (Position: M1-D216). Human GINS3 shares 89.4% amino acid (aa) sequence identity with mouse GINS3. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA replication complex GINS protein PSF3 (gene GINS3) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
GINS3 (PSF3) is a subunit of the four-protein GINS complex. GINS joins CDC45 and the MCM2–7 ring to build the CMG helicase, the motor that unwinds DNA at replication forks, which makes GINS3 relevant to studies of DNA replication and cell cycle progression.
| Target | DNA replication complex GINS protein PSF3 (gene GINS3; UniProt Q9BRX5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human GINS3 fragment (Met1–Asp216), expressed in E. coli. Sequence identity with the mouse ortholog: 89.4%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA replication complex GINS protein PSF3 in the nucleus and chromosome.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
