| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gap junction gamma-2 protein;Connexin-46.6;Cx46.6;Connexin-47;Cx47;Gap junction alpha-12 protein;GJC2;GJA12; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GJC2, different from the related mouse and rat sequences by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes gap junction gamma-2 protein (gene GJC2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 47.0 kDa, and the supplier reports an observed band at about 47 kDa.
Connexin 47 (GJC2) is a gap junction protein expressed mainly in oligodendrocytes. It forms channels with astrocytic connexins that link glial cells and supports myelination in the central nervous system, and GJC2 mutations are known to cause a hypomyelinating leukodystrophy.
| Target | Gap junction gamma-2 protein (gene GJC2; UniProt Q5T442, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 47.0 kDa |
| Observed MW | 47 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, U2OS cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human GJC2. It differs from the mouse and rat sequences at five residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places gap junction gamma-2 protein in the cell membrane and cell junction. Tissue expression noted by UniProt (human): Expressed in central nervous system, in sciatic nerve and sural nerve. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
