| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GK5 recombinant protein (Position: D37-K402). Human GK5 shares 82.2% amino acid (aa) sequence identity with mouse GK5. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GK5 Antibody Picoband® is an antibody targeting GK5. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: IgG; reactivity: Human; observed MW: 59 kDa; calculated MW: 59 kDa.
Boster Bio Anti-GK5 Antibody Picoband® catalog # A15997. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: GK5
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 59 kDa; Calculated: 59 kDa
Biological background
Scientific background (datasheet): Glycerol kinase 5 (GK5), encoded by the GK5 gene, is a member of the glycerol kinase family involved in glycerol metabolism. While its specific functions are still being elucidated, GK5 is implicated in various physiological processes such as energy metabolism and lipid homeostasis, warranting further investigation into its role and potential implications in health and disease.
Research relevance and current trends
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
