| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beta-galactosidase; Glb1; Glb1_mapped; rCG_25043 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat GLB1/Beta-galactosidase recombinant protein (Position: R103-I646). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets beta-galactosidase (gene Glb1) in mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
Beta-galactosidase (GLB1) is a lysosomal enzyme that removes terminal beta-linked galactose from gangliosides, glycoproteins and glycosaminoglycans. A spliced isoform lacks enzyme activity and serves as the elastin-binding protein that helps assemble elastic fibers. GLB1 deficiency causes GM1 gangliosidosis and Morquio B disease, and its lysosomal activity underlies the senescence-associated β-galactosidase marker.
| Target | Beta-galactosidase (gene Glb1; UniProt D3ZUM4, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Observed MW | 65 kDa, 76 kDa, 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, rat liver tissue, rat testis tissue, mouse liver tissue, mouse testis tissue, mouse HEPA1–6 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): NRK cells.
Samples with a confirmed signal (flow cytometry): HEPA 1–6 cells, RH35 cells.
Immunogen
Recombinant rat GLB1/Beta-galactosidase fragment (Arg103–Ile646), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places beta-galactosidase in the lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hu Hongtu et al. (2025) Impaired glycolysis-derived serine metabolism as a key driver of podocyte injury with senescence. Nature Communications. 10.1038/s41467-025-66850-1
