| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription activator GLI1; Glioma-associated oncogene; Oncogene GLI; GLI1; GLI |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057). Human GLI1 shares 79.8% |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transcription activator GLI1 in human, mouse and rat samples and is validated for Western blot and ELISA.
GLI1 is a zinc finger transcription factor that acts as an activator at the end of the Hedgehog signaling pathway. When Hedgehog ligands relieve Smoothened inhibition, GLI1 enters the nucleus and switches on target genes, including itself, so it is widely used as a readout of Hedgehog pathway activity in development and cancer research.
| Target | Transcription activator GLI1 (gene GLI1; UniProt P08151, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human GLI1 fragment (Tyr800–Gln1057), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription activator GLI1 in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Detected in testis (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
