| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutaminase liver isoform, mitochondrial; GLS; L-glutaminase; L-glutamine amidohydrolase; GLS2; GA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GLS2 recombinant protein (Position: M1-V602). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects glutaminase liver isoform, mitochondrial (gene GLS2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 66.3 kDa, and the supplier reports an observed band at about 66 kDa.
Liver-type glutaminase (GLS2) is a mitochondrial enzyme that converts glutamine to glutamate, which can then feed α-ketoglutarate into the TCA cycle. It supports mitochondrial respiration and glutathione-based antioxidant defense and is a known p53 target gene, so it is studied in metabolism and tumor suppression.
| Target | Glutaminase liver isoform, mitochondrial (gene GLS2; UniProt Q9UI32, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 66.3 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U-87MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human GLS2 fragment (Met1–Val602), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places glutaminase liver isoform, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Highly expressed in liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Wang et al. (2022) Dysfunction of Cytotoxic T Lymphocyte Induced by Hepatoma Cells through the Gln-GLS2-Endoplasmic Reticulum Stress Pathway. Frontiers in Bioscience-Landmark. 10.31083/j.fbl2708243
