| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glucagon;Glicentin;Glicentin-related polypeptide;GRPP;Oxyntomodulin;OXM;OXY;Glucagon;Glucagon-like peptide 1;GLP-1;Incretin hormone;Glucagon-like peptide 1 (7-37);GLP-1 (7-37);Glucagon-like peptide 1 (7-36);GLP-1 (7-36);Glucagon-like peptide 2;GLP-2;GCG; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Glucagon |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOAF-7 is a rabbit monoclonal antibody that recognizes pro-glucagon (gene GCG) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 20.9 kDa, and the supplier reports an observed band at about 21 kDa.
Proglucagon (GCG) is processed into several hormones depending on the tissue. In pancreatic α cells it yields glucagon, which raises blood glucose by stimulating hepatic glucose output, and in intestinal L cells it yields GLP-1 and GLP-2, which boost insulin secretion and support gut growth. GCG products are central to studies of glucose homeostasis, diabetes and obesity.
| Target | Pro-glucagon (gene GCG; UniProt P01275, human) |
|---|---|
| Clone | AOAF-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.9 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse pancrease tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human glucagon.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes pro-glucagon as a secreted protein. Tissue expression noted by UniProt (human): [Glucagon]: Secreted in the A cells of the islets of Langerhans... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gaoxing Ma et al. (2018) Impacts of Dietary Pleurotus eryngii Polysaccharide on Nutrient Digestion, Metabolism, and Immune Response of the Small Intestine and Colon—An iTRAQ-Based Proteomic Analysis. Proteomics. 10.1002/pmic.201700443
