| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glucocorticoid receptor;GR;Nuclear receptor subfamily 3 group C member 1;NR3C1;GRL; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NR3C1 recombinant protein (Position: M1-D373). Human NR3C1 shares 85% and 82% amino acid (aa) sequences identity with mouse and rat NR3C1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glucocorticoid receptor (gene NR3C1) in human samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 85.7 kDa, and the supplier reports an observed band at about 95 kDa.
The glucocorticoid receptor (GR, NR3C1) is a ligand-activated nuclear receptor for cortisol and synthetic glucocorticoids. On hormone binding it moves to the nucleus, binds glucocorticoid response elements and also restrains other transcription factors such as NF-κB and AP-1. GR mediates anti-inflammatory, metabolic and stress responses and is widely studied in endocrinology and immunology.
| Target | Glucocorticoid receptor (gene NR3C1; UniProt P04150, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 85.7 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human gastric cancer tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human NR3C1 fragment (Met1–Asp373), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 85% and 82%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiao Li et al. (2022) CircDYM ameliorates CUMS mice depressive-like behavior and inhibits hippocampal neurons injury via miR-497a-5p/NR3C1 axis. Brain Research. 10.1016/j.brainres.2022.147911
