| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glucose-6-phosphate isomerase;GPI;5.3.1.9;Autocrine motility factor;AMF;Neuroleukin;NLK;Phosphoglucose isomerase;PGI;Phosphohexose isomerase;PHI;Sperm antigen 36;SA-36;GPI; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human GPI, different from the related mouse and rat sequences by sixteen amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glucose-6-phosphate isomerase (gene GPI) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 63.1 kDa, and the supplier reports an observed band at about 63 kDa.
Glucose-6-phosphate isomerase (GPI) interconverts glucose 6-phosphate and fructose 6-phosphate, the second step of glycolysis and a reverse step in gluconeogenesis. Outside the cell the same protein acts as a cytokine known as autocrine motility factor or neuroleukin, and GPI deficiency causes hemolytic anemia.
| Target | Glucose-6-phosphate isomerase (gene GPI; UniProt P06744, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 63.1 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human SiHa cells, human A549 cells, human K562 cells, human A431 cells, rat lung tissue, rat heart tissue, mouse lung tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Synthetic peptide from the N-terminal region of human GPI. It differs from the mouse and rat sequences at sixteen residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes glucose-6-phosphate isomerase as secreted and also located in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
