| Field | Specification |
|---|---|
| Target | |
| Alternative names | Solute carrier family 2, facilitated glucose transporter member 4;Glucose transporter type 4, insulin-responsive;GLUT-4;SLC2A4;GLUT4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects solute carrier family 2, facilitated glucose transporter member 4 (gene SLC2A4) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 54.8 kDa, and the supplier reports an observed band at about 55 kDa.
GLUT4 (SLC2A4) is the insulin-responsive glucose transporter of skeletal muscle, heart and fat cells. Without insulin it is held in intracellular vesicles, and insulin or muscle contraction moves it to the plasma membrane to boost glucose uptake, so GLUT4 trafficking is a central topic in insulin resistance and diabetes research.
| Target | Solute carrier family 2, facilitated glucose transporter member 4 (gene SLC2A4; UniProt P14672, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 54.8 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
Samples with a confirmed band (WB): rat cardiac muscle tissue, rat skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, rat cardiac muscle tissue, mouse skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): rat cardiac muscle tissue, mouse cardiac muscle tissue.
Immunogen
Recombinant human GLUT4 fragment (Asn333–Asp509), expressed in E. coli. Sequence identity with the mouse ortholog: 97%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places solute carrier family 2, facilitated glucose transporter member 4 in the cell membrane, endomembrane system and cytoplasm. Tissue expression noted by UniProt (human): Skeletal and cardiac muscles. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiao Han et al. (2022) Aerobic exercise ameliorates insulin resistance in C57BL/6 J mice via activating Sestrin3. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2022.166568
Jing Zhou et al. (2023) Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice. Life Sciences. 10.1016/j.lfs.2023.121491
Yang Dai et al. (2022) Asiatic acid protests against myocardial ischemia/reperfusion injury via modulation of glycometabolism in rat cardiomyocyte. Drug Design Development and Therapy. 10.2147/DDDT.S175116
Xiaoyu Chen et al. (2022) Ameliorated effects of a lipopeptide surfactin on insulin resistance in vitro and in vivo. Food Science & Nutrition. 10.1002/fsn3.2852
Li Wang et al. (2016) Microwave-assisted synthesis of chitooligosaccharide guanidine and its effect on GLUT4-dependent glucose uptake through an Akt-activated protein kinase signaling pathway in L6 skeletal muscle cells. RSC Advances. 10.1039/C6RA17654B
Zejun Mo et al. (2019) Coumarins ameliorate diabetogenic action of dexamethasone via Akt activation and AMPK signaling in skeletal muscle. Journal of Pharmacological Sciences. 10.1016/j.jphs.2019.01.001
Zhang Xiaoli et al. (2022) The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway. Bioscience Biotechnology and Biochemistry. 10.1093/bbb/zbac103
Deng-Shen Zhang et al. (2019) Role of Phosphorylated AMP-Activated Protein Kinase (AMPK) in Myocardial Insulin Resistance After Myocardial Ischemia-Reperfusion During Cardiopulmonary Bypass Surgery in Dogs. Medical Science Monitor. 10.12659/MSM.916517
Thaís C. Costa et al. (2019) Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births. Animal Reproduction Science. 10.1016/j.anireprosci.2019.05.006
Xu Lei et al. (2024) Alpha-lipoic Acid Prevents Bone Loss in Type 2 Diabetes and Postmenopausal Osteoporosis Coexisting Conditions by Modulating the YAP/Glut4 Pathway. Cell Biochemistry and Biophysics. 10.1007/s12013-024-01216-w
