| Field | Specification |
|---|---|
| Target | |
| Alternative names | Solute carrier family 2, facilitated glucose transporter member 4;Glucose transporter type 4, insulin-responsive;GLUT-4;SLC2A4;GLUT4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GLUT4, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets solute carrier family 2, facilitated glucose transporter member 4 (gene SLC2A4) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 54.8 kDa, and the supplier reports an observed band at about 55 kDa.
GLUT4 (SLC2A4) is the insulin-responsive glucose transporter of skeletal muscle, heart and fat cells. Without insulin it is held in intracellular vesicles, and insulin or muscle contraction moves it to the plasma membrane to boost glucose uptake, so GLUT4 trafficking is a central topic in insulin resistance and diabetes research.
| Target | Solute carrier family 2, facilitated glucose transporter member 4 (gene SLC2A4; UniProt P14672, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 54.8 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat, Mouse) |
Samples with a confirmed band (WB): rat cardiac muscle tissue, rat skeletal muscle tissue, mouse cardiac muscle tissue, mouse skeletal muscle tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat cardiac muscle tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human GLUT4. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places solute carrier family 2, facilitated glucose transporter member 4 in the cell membrane, endomembrane system and cytoplasm. Tissue expression noted by UniProt (human): Skeletal and cardiac muscles. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Antonia Mortsch et al. (2026) Upregulation of miR-146a-5p and miR-146b-5p limits IL-1β-mediated signaling in adipose tissue during polytrauma by downregulating IRAK1. Frontiers in Immunology. 10.3389/fimmu.2026.1658504
Yulong Shang et al. (2014) Activation of κ-Opioid Receptor Exerts the Glucose-Homeostatic Effect in Streptozotocin-Induced Diabetic Mice. Journal of Cellular Biochemistry. 10.1002/jcb.24962
Wang Lu et al. (2019) Phenotypic characterization of a novel type 2 diabetes animal model in a SHANXI MU colony of Chinese hamsters. Endocrine. 10.1007/s12020-019-01940-x
Tian‑Jie Han et al. (2018) Leptin and its receptor in glucose metabolism of T‑cell lymphoma. Oncology Letters. 10.3892/ol.2018.9356
