| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutamate receptor 2; GluR-2; AMPA-selective glutamate receptor 2; GluR-B; GluR-K2; Glutamate receptor ionotropic, AMPA 2; GRIA2; GluA2; GLUR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GluR2 Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
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| Concentration | |
| Form | Liquid |
| Storage | |
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Product Overview
Clone ACIB-7 is a rabbit monoclonal antibody that recognizes glutamate receptor 2 (gene GRIA2) in human, mouse, rat and pig samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 98.8 kDa, and the supplier reports an observed band at about 99 kDa.
GluA2 (GRIA2) is a subunit of AMPA-type glutamate receptors. After RNA editing at the Q/R site, GluA2 makes AMPA receptors nearly impermeable to Ca2+, so its presence shapes excitatory synaptic transmission, plasticity and neuronal vulnerability to excitotoxicity.
| Target | Glutamate receptor 2 (gene GRIA2; UniProt P42262, human) |
|---|---|
| Clone | ACIB-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat, Pig |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 98.8 kDa |
| Observed MW | 99 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, pig brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human gluR2 Ionotropic glutamate receptor.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and pig. UniProt places glutamate receptor 2 in the cell membrane, postsynaptic cell membrane and postsynaptic density membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Song-Ji Li et al. (2023) CNTN1 Aggravates Neuroinflammation and Triggers Cognitive Deficits in Male Mice by Boosting Crosstalk between Microglia and Astrocytes. Aging and Disease. 10.14336/AD.2023.0228
Xiao-Yu Lu et al. (2024) Oral edaravone ameliorates behavioral deficits and pathologies in a valproic acid-induced rat model of autism spectrum disorder. Neuropharmacology. 10.1016/j.neuropharm.2024.110089
