| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutathione peroxidase 1;GPx-1;GSHPx-1;1.11.1.9;Cellular glutathione peroxidase;GPX1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human GPX1, different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glutathione peroxidase 1 (gene GPX1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 22 kDa.
Glutathione peroxidase 1 (GPX1) is a widely expressed selenoprotein that uses glutathione to reduce hydrogen peroxide and organic hydroperoxides. As one of the main cellular antioxidant enzymes, it protects cells from oxidative damage and helps set redox signaling tone, so it is common in oxidative stress research.
| Target | Glutathione peroxidase 1 (gene GPX1; UniProt P07203, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 22.1 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): human THP-1 cells, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human GPX1. It differs from the mouse sequence at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places glutathione peroxidase 1 in the cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Expressed in platelets (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yi Liu et al. (2020) Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2020.110321
Takashi Mori et al. (2020) Gallic acid is a dual α/β-secretase modulator that reverses cognitive impairment and remediates pathology in Alzheimer mice. Journal of Biological Chemistry. 10.1074/jbc.RA119.012330
Takashi Mori et al. (2019) Combined treatment with the phenolics (−)-epigallocatechin-3-gallate and ferulic acid improves cognition and reduces Alzheimer-like pathology in mice. Journal of Biological Chemistry. 10.1074/jbc.RA118.004280
Yingfeng Yang et al. (2018) The orphan nuclear receptor NR4A1 attenuates oxidative stress-induced β cells apoptosis via up-regulation of glutathione peroxidase 1. Life Sciences. 10.1016/j.lfs.2018.04.027
