| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from glutathione peroxidase 3 (GPx-P) protein in mouse, rat, orangutan and bovine. |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
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Product Overview
This polyclonal antibody, raised in Rabbit, recognizes GPX3. This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P). It is validated for Western Blot, Immunohistochemistry.
GPx-P belongs to the glutathione peroxidase family which are responsible for the detoxification of hydrogen peroxide. It protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPx-P is secreted in plasma and exists as a homotetramer.
Background
GPx-P belongs to the glutathione peroxidase family which are responsible for the detoxification of hydrogen peroxide. It protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPx-P is secreted in plasma and exists as a homotetramer.
Validated Applications
| Applications | Western Blot, Immunohistochemistry |
|---|
This is an extremely high titre antibody that detects 500 ng of peptide antigen on western blotting up to a dilution of 1: 500000. A dilution o f 1: 1000 to 1: 5000 is recommended for immunohistochemistry depending on detection method but end user will need to determine dilution to suit application. Antigen retrieval is recommended. For Western blotting it is recommended that several dilutions be tested in the range 1: 5000 to 1: 50000 although higher dilutions could be use if the antigen is in abundance in sample. The optimal dilution should be determined by the end user.
Immunogen
A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from glutathione peroxidase 3 (GPx-P) protein in mouse, rat, orangutan and bovine.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Human, Rat |
| Immunogen | A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from glutathione peroxidase 3 (GPx-P) protein in mouse, rat, orangutan and bovine. |
| Purification | Filter purified |
| Specificity | This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P). |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Storage Buffer | PBS, pH 7.4 with 0.02% sodium azide. |
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Shipping | Ice Pack |
Reactivity Notes
This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P).
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. Contains sodium azide where stated; handle with standard laboratory precautions.
Related Products
Explore related antibodies and detection reagents for GPX3 research from Applied Biological Materials (abm) Inc., or contact us for alternative conjugates and formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
