| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanine nucleotide-binding protein G; i; subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein; GNAI2; GNAI2B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GNAI2 recombinant protein (Position: R67-N312). Human GNAI2 shares 97.6% and 98% amino acid (aa) sequence identity with mouse and rat GNAI2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets guanine nucleotide-binding protein G(i) subunit alpha-2 (gene GNAI2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 40.5 kDa, and the supplier reports an observed band at about 40 kDa.
Gαi2 (GNAI2) is the alpha subunit of an inhibitory G protein. When activated by G protein-coupled receptors it exchanges GDP for GTP and inhibits adenylyl cyclase, lowering cAMP, and its GTPase activity then turns the signal off. It is studied in immune cell migration, cardiac signaling and hormone regulation.
| Target | Guanine nucleotide-binding protein G(i) subunit alpha-2 (gene GNAI2; UniProt P04899, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.5 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U-87MG cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human endometrioid adenocarcinoma tissue, human liver cancer tissue, human placenta tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
Recombinant human GNAI2 fragment (Arg67–Asn312), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97.6% and 98%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places guanine nucleotide-binding protein G(i) subunit alpha-2 in the cytoplasm, cell membrane and membrane. Tissue expression noted by UniProt (human): Highly expressed in endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
