| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanine nucleotide-binding protein G; q; subunit alpha; Guanine nucleotide-binding protein alpha-q; GNAQ; GAQ |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GNAQ |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30G74 is a rabbit monoclonal antibody that recognizes guanine nucleotide-binding protein G(q) subunit alpha (gene GNAQ) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 42.1 kDa, and the supplier reports an observed band at about 40 kDa.
Gαq (GNAQ) is the alpha subunit of Gq heterotrimeric G proteins. After receptor activation it stimulates phospholipase C-β, producing IP3 and diacylglycerol that release Ca2+ and activate protein kinase C. Activating GNAQ mutations occur in uveal melanoma and Sturge-Weber syndrome, making it a common research target.
| Target | Guanine nucleotide-binding protein G(q) subunit alpha (gene GNAQ; UniProt P50148, human) |
|---|---|
| Clone | 30G74 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.1 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human HepG2 cells, human U251 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human testis tissue, mouse testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human GNAQ.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places guanine nucleotide-binding protein G(q) subunit alpha in the cell membrane, Golgi apparatus and nucleus. Tissue expression noted by UniProt (human): Predominantly expressed in ovary, prostate, testis and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
