| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein lin-28 homolog B;Lin-28B;LIN28B;CSDD2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human GNAQ |
| Isotype | |
| Molecular Weight | |
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| Reconstitution | |
| Target | |
| UniProt # |
Boster Bio Anti-GNAQ Rabbit Monoclonal Antibody catalog # M00898-1. Tested in IHC, WB application. This antibody reacts with Human, Mouse, Rat.
Protein: Glycogen synthase kinase-3 beta (gsk3b) | Gene: Protein lin-28 homolog B | Target: GNAQ | Function: Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11- independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. . | Research areas: Cancer,Epigenetics and Nuclear Signaling,Tumor Biomarkers
Host: Rabbit | Clonality/Clone: Monoclonal / Clone: 30G74 | Isotype: IgG | Reactivity: Human,Mouse,Rat | Immunogen: A synthesized peptide derived from human GNAQ | Concentration: 0.5mg/ml | Purification: Affinity-chromatography
Applications: WB, IHC, IF, ICC
Observed MW: 42 kDa | Calculated MW: 27084 MW
Cellular localization: Nucleus. Nucleus, nucleolus . Cytoplasm . Predominantly nucleolar (PubMed:22118463). In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064). .
Tissue details: Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064). Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed:22118463). .
Status: Active
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