| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Guanine nucleotide-binding protein G (I)/G (S)/G (T) subunit beta-1;Transducin beta chain 1;GNB1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human GNB1, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GNB1 (Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GNB1 Antibody Picoband® catalog # PB10067. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human GNB1, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 37 kDa; calculated MW: 37377 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, IHC-F, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1. Guanine nucleotide-binding protein G (I)/G (S)/G (T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. Functional note: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Reported localization: Cytoplasm, cell cortex . Cytoplasm, myofibril, sarcomere, Z line . Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3 (By similarity). . Expression/tissue context: Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.
Research relevance and current trends
- Cancer: Researchers commonly examine how GNB1 (Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how GNB1 (Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) relates to this theme using model systems and orthogonal readouts.
- Energy Transfer Pathways: Researchers commonly examine how GNB1 (Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GNB1 (Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of GNB1 (Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
