| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; GNE; GLCNE; IBM2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GNE |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29G96 is a rabbit monoclonal antibody that recognizes bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (gene GNE) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 79.3 kDa, and the supplier reports an observed band at about 75 kDa.
GNE is a bifunctional enzyme that acts both as an epimerase on UDP-GlcNAc and as a kinase on the resulting N-acetylmannosamine. It carries out the committed, rate-limiting step in making sialic acid, the negatively charged sugar that caps many cell surface glycans, and GNE mutations cause GNE myopathy, so it is studied in glycobiology and muscle disease.
| Target | Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (gene GNE; UniProt Q9Y223, human) |
|---|---|
| Clone | 29G96 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 79.3 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GNE.
Reactivity Notes
The supplier lists reactivity with human. UniProt places bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in the cytoplasm. Tissue expression noted by UniProt (human): Highest expression in liver and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
