| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanine nucleotide-binding protein G; I; /G; S; O; subunit gamma-4; GNG4; GNGT4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GNG4 recombinant protein (Position: M1-D52). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 7B6 is a mouse monoclonal antibody that recognizes guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 (gene GNG4) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 8.4 kDa, and the supplier reports an observed band at about 12 kDa.
Gγ4 (GNG4) is a gamma subunit of heterotrimeric G proteins. Bound to a Gβ subunit, it forms the Gβγ dimer that is required for G protein activation and relays signals from G protein-coupled receptors to downstream effectors; it is expressed notably in the brain.
| Target | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 (gene GNG4; UniProt P50150, human) |
|---|---|
| Clone | 7B6 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 8.4 kDa |
| Observed MW | 12 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human GNG4 fragment (Met1–Asp52), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 in the cell membrane. Tissue expression noted by UniProt (human): Brain, kidney, pancreas, skeletal muscle and faintly in cardiac muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
