| Field | Specification |
|---|---|
| Target | |
| Alternative names | N-acetylglucosamine-6-sulfatase; Glucosamine-6-sulfatase; G6S; GNS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human GNS recombinant protein (Position: W238-R355). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets N-acetylglucosamine-6-sulfatase (gene GNS) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 62.1 kDa, and the supplier reports an observed band at about 90 kDa.
N-acetylglucosamine-6-sulfatase (GNS) is a lysosomal enzyme that removes 6-sulfate groups from N-acetylglucosamine units of heparan sulfate and keratan sulfate during their breakdown. GNS deficiency causes mucopolysaccharidosis type IIID (Sanfilippo syndrome D), so it is studied in lysosomal storage disorders.
| Target | N-acetylglucosamine-6-sulfatase (gene GNS; UniProt P15586, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 62.1 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human PANC-1 cells, human SGC-7901 cells, rat lung tissue, rat kidney tissue, rat testis tissue, rat PC-12 cells, mouse lung tissue, mouse kidney tissue, mouse testis tissue, mouse spleen tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human GNS fragment (Trp238–Arg355), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places N-acetylglucosamine-6-sulfatase in the lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
