| Field | Specification |
|---|---|
| Target | |
| Alternative names | Golgi integral membrane protein 4; Golgi integral membrane protein, cis; GIMPc; Golgi phosphoprotein 4; Golgi-localized phosphoprotein of 130 kDa; Golgi phosphoprotein of 130 kDa; GOLIM4; GOLPH4; GPP130 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GOLPH4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21G09 is a rabbit monoclonal antibody that recognizes golgi integral membrane protein 4 (gene GOLIM4) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 81.9 kDa, and the supplier reports an observed band at about 130 kDa.
GOLIM4 (also called GPP130) is a type II membrane protein of the cis-Golgi that cycles between the Golgi and endosomes. It follows a route from early endosomes directly to the Golgi that bypasses late endosomes, and this pathway is used by Shiga toxin, so it is studied in membrane trafficking.
| Target | Golgi integral membrane protein 4 (gene GOLIM4; UniProt O00461, human) |
|---|---|
| Clone | 21G09 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 81.9 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GOLPH4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places golgi integral membrane protein 4 in the Golgi apparatus, endosome membrane and membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
