| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pancreatic secretory granule membrane major glycoprotein GP2; Pancreatic zymogen granule membrane protein GP-2; Gp2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse GP2 recombinant protein (Position: S35-R497). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes pancreatic secretory granule membrane major glycoprotein GP2 in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 59.2 kDa, and the supplier reports an observed band at about 55 kDa.
In mouse, glycoprotein 2 (GP2) is a GPI-anchored protein found in pancreatic zymogen granules and on intestinal M cells. On M cells it acts as a receptor for FimH on type I-piliated bacteria, carrying them across the epithelium to Peyer's patches to start mucosal immune responses.
| Target | Pancreatic secretory granule membrane major glycoprotein GP2 (gene Gp2; UniProt Q9D733, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 59.2 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse kidney tissue, mouse brain tissue, mouse heart tissue, rat kidney tissue, rat brain tissue, rat heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat pancreas tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Neuro-2a cells.
Immunogen
Recombinant mouse GP2 fragment (Ser35–Arg497), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes pancreatic secretory granule membrane major glycoprotein GP2 as secreted and also located in the zymogen granule membrane, cell membrane and apical cell membrane. Tissue expression noted by UniProt (mouse): Specifically expressed by M (microfold) cells which are atypical epithelial cells of the intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wu Shijing et al. (2023) Effects of Intestinal M Cells on Intestinal Barrier and Neuropathological Properties in an AD Mouse Model. Molecular Neurobiology. 10.1007/s12035-023-03807-9
