| Field | Specification |
|---|---|
| Target | |
| Alternative names | G protein-coupled bile acid receptor 1; G protein-coupled receptor GPCR19; hGPCR19; Membrane-type receptor for bile acids; M-BAR; hBG37; BG37; GPBAR1; TGR5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR TGR5/GPBAR1, which shares 100% and 92.9% amino acid (aa) sequence identity with human and rat GPCR TGR5/GPBAR1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects G protein-coupled bile acid receptor 1 (gene GPBAR1) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 35.2 kDa, and the supplier reports an observed band at about 46–48 kDa.
GPBAR1 (also called TGR5) is a G protein-coupled receptor activated by bile acids, with lithocholic acid among the most potent. It couples to Gs to raise cAMP and is expressed in the intestine, gallbladder, immune cells and other tissues, where it helps regulate energy expenditure, GLP-1 release and inflammation.
| Target | G protein-coupled bile acid receptor 1 (gene GPBAR1; UniProt Q8TDU6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 35.2 kDa |
| Observed MW | 46–48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human GPCR TGR5/GPBAR1. Sequence identity with the human and rat orthologs: 100% and 92.9%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places G protein-coupled bile acid receptor 1 in the cell membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenjing Mo et al. (2023) Noni (Morinda citrifolia L.) fruit polysaccharide ameliorated high-fat diet-induced obesity by modulating gut microbiota and improving bile acid metabolism. Journal of Functional Foods. 10.1016/j.jff.2023.105408
