| Field | Specification |
|---|---|
| Target | |
| Alternative names | G protein-coupled receptor family C group 5 member B; A-69G12.1; Retinoic acid-induced gene 2 protein; RAIG-2; GPRC5B; RAIG2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GPRC5B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29G58 is a rabbit monoclonal antibody that recognizes G protein-coupled receptor family C group 5 member B (gene GPRC5B) in human samples and is validated for Western blot. The predicted molecular weight is 44.8 kDa, and the supplier reports an observed band at about 52 kDa.
GPRC5B is an orphan G protein-coupled receptor of family C that is highly expressed in the brain. It has been linked to regulation of cell volume and to metabolic and inflammatory signaling in adipose tissue, although its ligand and full function remain unclear.
| Target | G protein-coupled receptor family C group 5 member B (gene GPRC5B; UniProt Q9NZH0, human) |
|---|---|
| Clone | 29G58 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.8 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GPRC5B.
Reactivity Notes
The supplier lists reactivity with human. UniProt places G protein-coupled receptor family C group 5 member B in the cell membrane and cytoplasmic vesicle membrane. Tissue expression noted by UniProt (human): Expression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
