| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutathione peroxidase 1;GPx-1;GSHPx-1;1.11.1.9;Cellular glutathione peroxidase;GPX1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GPX1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IIF-7 is a rabbit monoclonal antibody that recognizes glutathione peroxidase 1 (gene GPX1) in human and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 22 kDa.
Glutathione peroxidase 1 (GPX1) is a widely expressed selenoprotein that uses glutathione to reduce hydrogen peroxide and organic hydroperoxides. As one of the main cellular antioxidant enzymes, it protects cells from oxidative damage and helps set redox signaling tone, so it is common in oxidative stress research.
| Target | Glutathione peroxidase 1 (gene GPX1; UniProt P07203, human) |
|---|---|
| Clone | IIF-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.1 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): SH SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GPX1.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places glutathione peroxidase 1 in the cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Expressed in platelets (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
