| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phospholipid hydroperoxide glutathione peroxidase GPX4; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4; GPX4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GPX4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACCO-7 is a rabbit monoclonal antibody that recognizes phospholipid hydroperoxide glutathione peroxidase GPX4 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 22.2 kDa, and the supplier reports an observed band at about 19 kDa.
Glutathione peroxidase 4 (GPX4) is a selenoprotein that reduces phospholipid hydroperoxides directly within membranes. By preventing lipid peroxidation, it protects cells from ferroptosis, an iron-dependent form of cell death, making GPX4 a central target in ferroptosis, neurodegeneration and cancer research.
| Target | Phospholipid hydroperoxide glutathione peroxidase GPX4 (gene GPX4; UniProt P36969, human) |
|---|---|
| Clone | ACCO-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.2 kDa |
| Observed MW | 19 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): mouse RAW264.7(WT) cells, mouse RAW264.7(KO) cells, human MCF-7 cells, human HepG2 cells, human Caco-2 cells, human 293T cells, human K562 cells, rat PC-12 cells, rat NRK cells, mouse RAW264.7 cells, mouse NIH/3T3 cells, rat kidney tissue, rat testis tissue, mouse kidney tissue, mouse testis tissue, human HCT116- WT cells, human HCT116-GPX4 KO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human GPX4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Present primarily in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongyang Jiang et al. (2026) Iterative-Designed Multifunctional Carbon Dots Nanodrug for Celastrol Loading, Targeting Delivery, and Combination Therapy of Kidney Stones. Advanced Functional Materials. 10.1002/adfm.202523181
Ke Li et al. (2022) Multienzyme-like Reactivity Cooperatively Impairs Glutathione Peroxidase 4 and Ferroptosis Suppressor Protein 1 Pathways in Triple-Negative Breast Cancer for Sensitized Ferroptosis Therapy. ACS Nano. 10.1021/acsnano.1c08664
Zeng Wenjun et al. (2025) Inhibition of ferroptosis counteracts the advanced maternal age-induced oocyte deterioration. Cell Death and Differentiation. 10.1038/s41418-025-01456-0
Chen Duan et al. (2024) Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation. Advanced Science. 10.1002/advs.202408945
Jiemei Chen et al. (2022) Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora. Pharmacological Research. 10.1016/j.phrs.2022.106191
Liu Na et al. (2026) SPOCK1 acts as a negative regulator of ferroptosis in non-small cell lung cancer. npj Precision Oncology. 10.1038/s41698-026-01455-5
Juan Sheng et al. (2026) Lycorine Suppresses DPP3-Mediated Nrf2 Signaling, Thereby Reducing SLC7A11 Levels to Trigger Ferroptosis in Colorectal Cancer Cells. Phytomedicine. 10.1016/j.phymed.2026.158221
Xin Fan et al. (2025) Celastrol Attenuates Ferroptosis-Mediated Intestinal Ischemia/Reperfusion-Induced Acute Lung Injury via Hippo-YAP Signaling. Phytomedicine. 10.1016/j.phymed.2025.156865
Tao Ding et al. (2025) Schisandrin B ameliorates Alzheimer's disease by suppressing neuronal ferroptosis and ensuing microglia M1 polarization. Phytomedicine. 10.1016/j.phymed.2025.156780
Yi Chen et al. (2026) IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2026.01.029
