| Field | Specification |
|---|---|
| Target | |
| Alternative names | Granzyme K; GrK; Fragmentin-3; Granzyme-3; NK-tryptase-2; NK-Tryp-2; GZMK; TRYP2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Granzyme K/GZMK, which shares 71.4% and 73.9% amino acid (aa) sequence identity with mouse and rat GZMK, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets granzyme K (gene GZMK) in human samples and is validated for IHC. The predicted molecular weight is 28.9 kDa.
Granzyme K (GZMK) is a tryptase-like serine protease made mainly by CD8+ T cells and some NK cells. Unlike granzymes A and B, it is largely secreted and has been shown to trigger a complement cascade that supports pathogen clearance and inflammation. GZMK-expressing T cells are studied in aging, chronic inflammation and tissue immunity.
| Target | Granzyme K (gene GZMK; UniProt P49863, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human granzyme K/GZMK. Sequence identity with the mouse and rat orthologs: 71.4% and 73.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes granzyme K as secreted and also located in the cell surface and cytoplasmic granule. Tissue expression noted by UniProt (human): Specifically expressed by CD8(+) T-cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
