| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beta-adrenergic receptor kinase 1;Beta-ARK-1;2.7.11.15;G-protein coupled receptor kinase 2;ADRBK1;BARK, BARK1, GRK2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GRK2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EGE-1 is a rabbit monoclonal antibody that recognizes G protein-coupled receptor kinase 2 (gene GRK2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 79.6 kDa, and the supplier reports an observed band at about 80 kDa.
GRK2 (also called βARK1 or ADRBK1) is a serine/threonine kinase that phosphorylates agonist-bound G protein-coupled receptors such as the β2-adrenergic receptor. This marks receptors for β-arrestin binding, which uncouples them from G proteins and drives internalization. GRK2 is widely expressed and is studied in receptor desensitization, cardiac function and heart failure.
| Target | G protein-coupled receptor kinase 2 (gene GRK2; UniProt P25098, human) |
|---|---|
| Clone | EGE-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 79.6 kDa |
| Observed MW | 80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GRK2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places G protein-coupled receptor kinase 2 in the cytoplasm, cell membrane, cell projection and postsynapse. Tissue expression noted by UniProt (human): Expressed in peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
