| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stress-70 protein, mitochondrial; mt-HSP70; 75 kDa glucose-regulated protein; GRP-75; Heat shock 70 kDa protein 9; Heat shock protein family A member 9; Mortalin; MOT; Peptide-binding protein 74; PBP74; HSPA9; GRP75; HSPA9B |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Grp75 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17H65 is a rabbit monoclonal antibody that recognizes stress-70 protein, mitochondrial (gene HSPA9) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 73.7 kDa, and the supplier reports an observed band at about 74 kDa.
Mortalin (HSPA9, also called GRP75 or mtHSP70) is the HSP70 chaperone of the mitochondrial matrix. It pulls proteins through the import machinery, helps fold them and supports iron-sulfur cluster assembly, and it also has roles outside mitochondria. It is studied in mitochondrial function, aging and cancer.
| Target | Stress-70 protein, mitochondrial (gene HSPA9; UniProt P38646, human) |
|---|---|
| Clone | 17H65 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 73.7 kDa |
| Observed MW | 74 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells, mouse liver.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human grp75.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places stress-70 protein, mitochondrial in the mitochondrion, nucleus, cytoplasm and mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
