| Field | Specification |
|---|---|
| Target | |
| Alternative names | 78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP;HSPA5;GRP78; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets endoplasmic reticulum chaperone BiP (gene HSPA5) in rat, mouse and human samples and is validated for Western blot and IHC. The predicted molecular weight is 72.3 kDa, and the supplier reports an observed band at about 78 kDa.
BiP (HSPA5, also called GRP78) is the main HSP70 chaperone of the endoplasmic reticulum lumen. It helps fold and assemble newly made secreted and membrane proteins, holds misfolded ones for degradation, and controls the unfolded protein response sensors IRE1, PERK and ATF6. It is a standard marker of ER stress.
| Target | Endoplasmic reticulum chaperone BiP (gene HSPA5; UniProt P11021, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 72.3 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.025–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human U251 cells, human HepG2 cells, human K562 cells, human A431 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.17 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human cerebral cortex tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human GRP78 BiP. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places endoplasmic reticulum chaperone BiP in the endoplasmic reticulum lumen, melanosome, cytoplasm and cell surface. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jicang Wang et al. (2022) Role of endoplasmic reticulum stress in cadmium-induced hepatocyte apoptosis and the protective effect of quercetin. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2022.113772
Yao Shu et al. (2015) RoY Peptide-Modified Chitosan-Based Hydrogel to Improve Angiogenesis and Cardiac Repair under Hypoxia. ACS Applied Materials & Interfaces. 10.1021/acsami.5b01234
Xiaoqin Zhang et al. (2022) Chimonanthus salicifolius attenuated vascular remodeling by alleviating endoplasmic reticulum stress in spontaneously hypertensive rats. Food & Function. 10.1039/D1FO04381A
Ke-xin Yan et al. (2024) Taraxasterol attenuates zearalenone-induced kidney damage in mice by modulating oxidative stress and endoplasmic reticulum stress. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2024.117093
Ding Lulu et al. (2023) Quercetin alleviates cadmium-induced BRL-3A cell apoptosis by inhibiting oxidative stress and the PERK/IRE1α/ATF6 signaling pathway. Environmental Science and Pollution Research. 10.1007/s11356-023-31189-x
Yuzhen Sui et al. (2024) BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress. CNS Neuroscience & Therapeutics. 10.1111/cns.14840
Ye Ding et al. (2011) Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect. PLoS One. 10.1371/journal.pone.0020008
Yan Huang et al. (2021) Elucidation of the mechanism of NEFA-induced PERK-eIF2α signaling pathway regulation of lipid metabolism in bovine hepatocytes. Journal of Steroid Biochemistry and Molecular Biology. 10.1016/j.jsbmb.2021.105893
Fangfang Wang et al. (2025) HNF4A Regulated APEH Deficiency Promotes UPR Activation in Diabetic Kidney Disease. FASEB Journal. 10.1096/fj.202500106R
Yumei Li et al. (2023) DJ-1 inhibits ferroptosis in cerebral ischemia-reperfusion via ATF4/HSPA5 pathway. Neurochemistry International. 10.1016/j.neuint.2023.105628
