| Field | Specification |
|---|---|
| Target | |
| Alternative names | 78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP;HSPA5;GRP78; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GRP78 BiP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BOO-8 is a rabbit monoclonal antibody that recognizes endoplasmic reticulum chaperone BiP (gene HSPA5) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 72.3 kDa, and the supplier reports an observed band at about 72 kDa.
BiP (HSPA5, also called GRP78) is the main HSP70 chaperone of the endoplasmic reticulum lumen. It helps fold and assemble newly made secreted and membrane proteins, holds misfolded ones for degradation, and controls the unfolded protein response sensors IRE1, PERK and ATF6. It is a standard marker of ER stress.
| Target | Endoplasmic reticulum chaperone BiP (gene HSPA5; UniProt P11021, human) |
|---|---|
| Clone | BOO-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 72.3 kDa |
| Observed MW | 72 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human non-Hodgkin's lymphoma tissue, human thyroid cancer tissue, human prostate cancer tissue, rat testis tissue, mouse skin tissue, human pancreas tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human GRP78 BiP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endoplasmic reticulum chaperone BiP in the endoplasmic reticulum lumen, melanosome, cytoplasm and cell surface. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cailian Wu et al. (2019) Effect of endoplasmic reticulum stress involved in manganese‑induced neurotoxicity in rats. Molecular Medicine Reports. 10.3892/mmr.2019.10175
