Anti-GRP94 HSP90B1 Rabbit Monoclonal Antibody

Overview

This product is an anti-HSP90B1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAHG-8; isotype Rabbit IgG; reactivity: Human,Mouse. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-GRP94 HSP90B1 Rabbit Monoclonal Antibody catalog # M02393-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Key elements and design rationale

• Target: HSP90B1 (Endoplasmin).
• Antibody format: Monoclonal; clone AAHG-8; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

HSP90B1 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. . Reported cellular localization context: Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue expression notes (as provided): Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cancer Metabolism,ER Proteins,Metabolism,Metabolism Processes,Organelles,Pathways and Processes,Protein Trafficking,Response To Hypoxia,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.

Workflow ideas (metafield): Validate HSP90B1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HSP90B1 expression by Western blot in cell or tissue lysates, Detect HSP90B1 in FFPE tissue sections by immunohistochemistry, Localize HSP90B1 by immunofluorescence/immunocytochemistry in cultured cells

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 60 kDa; calculated MW: 92469 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 60 kDa
• Cellular localization (provided): Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Tissue details (provided): Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.