Anti-GSDMD Antibody Picoband®

Overview

Anti-GSDMD Antibody Picoband® is an antibody for GSDMD detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: GSDMD (Melanocortin receptor 3); UniProt: P57764
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 30, 53 kDa, calculated 36043 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-GSDMD Antibody Picoband® catalog # A02842.

Biological background

Biological context: Receptor for MSH (alpha, beta and gamma) and ACTH. This receptor is mediated by G proteins which activate adenylate cyclase. Required for expression of anticipatory patterns of activity and wakefulness during periods of limited nutrient availability and for the normal regulation of circadian clock activity in the brain. .

Expression and localization notes: cellular localization: Cell membrane; Multi-pass membrane protein., tissue context: Brain, placental, and gut tissues..

Common research applications

• Western blotting (WB): Compare GSDMD levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of GSDMD in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify GSDMD-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane; Multi-pass membrane protein.
• Tissue details: Brain, placental, and gut tissues.
• Research category: Atherosclerosis,Cardiovascular,Fatty Acid Oxidation,Fatty Acids,Heart Disease,Lipid and Lipoprotein Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Neurology Process,Neuroscience,Pathways and Processes,Redox Metabolism,Vascular Inflammation

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.