| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gasdermin-D; Gasdermin domain-containing protein 1; GSDMD; DFNA5L; GSDMDC1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GSDMD recombinant protein (Position: M1-H484). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 3D5 is a mouse monoclonal antibody that recognizes gasdermin-D (gene GSDMD) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 52.8 kDa, and the supplier reports an observed band at about 53 kDa.
Gasdermin D (GSDMD) is the main executor of pyroptosis, an inflammatory form of programmed cell death. Cleavage by inflammatory caspases such as caspase-1 and caspase-11 (caspase-4/5 in humans) frees its N-terminal fragment, which inserts into membranes and forms large pores. These pores release IL-1β and IL-18 and cause cell lysis, making GSDMD central to inflammasome and innate immunity research.
| Target | Gasdermin-D (gene GSDMD; UniProt P57764, human) |
|---|---|
| Clone | 3D5 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.8 kDa |
| Observed MW | 53 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human THP-1 cells, human SW620 cells, human Jurkat cells, human U87 cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human esophagus tissue, human liver cancer tissue, human lymph nodes tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human GSDMD fragment (Met1–His484), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places gasdermin-D in the [Gasdermin-D]: Cytoplasm and inflammasome. Tissue expression noted by UniProt (human): Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yongshu Fu et al. (2024) Penicilazaphilone C alleviates allergic airway inflammation and improves the immune microenvironment by hindering the NLRP3 inflammasome. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2024.116788
