| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GSK3 beta/GSK3B recombinant protein (Position: M1-T420). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glycogen synthase kinase-3 beta (gene GSK3B) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 46.7 kDa, and the supplier reports an observed band at about 46 kDa.
Glycogen synthase kinase-3 beta (GSK3B) is a widely expressed, constitutively active serine/threonine kinase that is inhibited by insulin, growth factor and Wnt signals. Its substrates include glycogen synthase, β-catenin, tau and several transcription factors, placing it at the crossroads of metabolism, Wnt signaling and cytoskeletal regulation. GSK3B is heavily studied in diabetes, neurodegeneration, mood disorders and cancer.
| Target | Glycogen synthase kinase-3 beta (gene GSK3B; UniProt P49841, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.7 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U-87MG cells, human HeLa cells, rat PC-12 cells, rat C6 cells, mouse RAW264.7 whole cel, mouse Neuro-2a whole cel.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human ovarian cancer tissue, human endometrial adenocarcinoma tissue, human spleen tissue, mouse brain tissu, rat brain tissue, mouse kidney tissue, human appendix tissue, human stomach cancer tissue, human endometrial cancer tissue, human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): human appendix tissue, rat testis tissue, mouse kidney tissue, human stomach cancer tissue, human colon cancer tissue, human pancreas cancer tissue.
Immunogen
Recombinant human GSK3 beta/GSK3B fragment (Met1–Thr420), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places glycogen synthase kinase-3 beta in the cytoplasm, nucleus and cell membrane. Tissue expression noted by UniProt (human): Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yi Chen et al. (2026) IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2026.01.029
Sihan Feng et al. (2025) Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study. Pharmaceuticals. 10.3390/ph18030345
Kuangyu Jin et al. (2026) STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT. Cancer Medicine. 10.1002/cam4.71862
Qi Qiu et al. (2023) Naringin Protects against Tau Hyperphosphorylation in Aβ25–35-Injured PC12 Cells through Modulation of ER, PI3K/AKT, and GSK-3β Signaling Pathways. Behavioural Neurology. 10.1155/2023/1857330
Hao-Nan Ding et al. (2025) Effects of bergapten-loaded long-circulating liposomes on osteogenic differentiation of human dental pulp stem cells. Journal of Asian Natural Products Research. 10.1080/10286020.2025.2575989
