| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glutathione S-transferase P;2.5.1.18;GST class-pi;GSTP1-1;GSTP1;FAEES3, GST3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human GST3 / GST pi Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-GSTP1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFDG-7; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-GST3 / GSTP1 Monoclonal Antibody catalog # M00394-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: GSTP1 (Glutathione S-transferase P).
- Antibody format: Monoclonal; clone AFDG-7; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
GSTP1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. . Reported cellular localization context: Cytoplasm . Mitochondrion . Nucleus . The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization. Tissue expression notes (as provided): Released primarily from activated T lymphocytes.
Research relevance and current trends
- Research context keywords from the source record include: Neurogenesis,Neurology Process,Neuroscience,Oxidative Stress,Pathways and Processes,Redox Metabolism.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate GSTP1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect GSTP1 expression by Western blot in cell or tissue lysates, Detect GSTP1 in FFPE tissue sections by immunohistochemistry, Localize GSTP1 by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 43 kDa; calculated MW: 23356 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 43 kDa
- Cellular localization (provided): Cytoplasm . Mitochondrion . Nucleus . The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.
- Tissue details (provided): Released primarily from activated T lymphocytes.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
