| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutathione S-transferase P;2.5.1.18;GST class-pi;GSTP1-1;GSTP1;FAEES3, GST3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GST3 / GST pi Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFDG-7 is a rabbit monoclonal antibody that recognizes glutathione S-transferase P (gene GSTP1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 23.4 kDa, and the supplier reports an observed band at about 23 kDa.
Glutathione S-transferase P (GSTP1, GST-pi) is a pi-class GST that conjugates glutathione to electrophilic compounds, including the lipid peroxidation product 4-hydroxynonenal. Beyond detoxification, it binds and restrains stress kinases such as JNK. GSTP1 is abundant in many tissues and tumors and is studied in drug resistance, oxidative stress and ferroptosis.
| Target | Glutathione S-transferase P (gene GSTP1; UniProt P09211, human) |
|---|---|
| Clone | AFDG-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.4 kDa |
| Observed MW | 23 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): PC3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GST3 / GST pi Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places glutathione S-transferase P in the cytoplasm, mitochondrion and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
