| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutathione S-transferase A4; GST class-alpha member 4; Glutathione S-transferase A4-4; GSTA4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human GSTA4 recombinant protein (Position: E29-P222). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets glutathione S-transferase A4 (gene GSTA4) in human and monkey samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 25.7 kDa, and the supplier reports an observed band at about 26 kDa.
Glutathione S-transferase A4 (GSTA4) is an alpha-class GST specialized for detoxifying lipid peroxidation products. It is especially active toward reactive aldehydes like 4-hydroxynonenal (4-HNE), linking them to glutathione, limiting damage from these reactive aldehydes. GSTA4 is studied in oxidative stress, lipid peroxidation and metabolic disease models.
| Target | Glutathione S-transferase A4 (gene GSTA4; UniProt O15217, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.005 mg NaN3 |
| Calculated MW | 25.7 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Monkey) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human SH-SY5Y, human HepG2 cells, human HEK293 cells, monkey skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human GSTA4 fragment (Glu29–Pro222), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and monkey. UniProt places glutathione S-transferase A4 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
