| Field | Specification |
|---|---|
| Target | |
| Alternative names | GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;GTPase HRas, N-terminally processed;HRAS;HRAS1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GTPase HRAS, identical to the related mouse and rat sequences. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets GTPase HRas in human, mouse and rat samples and is validated for IHC and Western blot.
HRAS is one of the three classic Ras small GTPases. It cycles between an active GTP-bound and an inactive GDP-bound state and passes signals from growth factor receptors to the RAF-MEK-ERK and PI3K pathways that control proliferation and survival. Activating HRAS mutations occur in some tumors and cause Costello syndrome.
| Target | GTPase HRas (gene HRAS; UniProt P01112, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human GTPase HRAS. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places GTPase HRas in the cell membrane, Golgi apparatus and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qiang Zuo et al. (2010) Development of cetuximab-resistant human nasopharyngeal carcinoma cell lines and mechanisms of drug resistance. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2010.03.003
Qiang Zuo et al. (2011) The Ras signaling pathway mediates cetuximab resistance in nasopharyngeal carcinoma. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2011.02.005
Ke-Wen Zhao et al. (2004) Integrin beta4 mAb inhibited apoptosis induced by deprivation of growth factors in vascular endothelial cells. ACTA Pharmacologica Sinica. PMID: 15169624
