| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beta-glucuronidase; Beta-G1; GUSB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GUSB |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25G35 is a rabbit monoclonal antibody that recognizes beta-glucuronidase (gene GUSB) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 74.7 kDa, and the supplier reports an observed band at about 75 kDa.
Beta-glucuronidase (GUSB) is a lysosomal enzyme that removes glucuronic acid residues from glycosaminoglycans such as dermatan sulfate, heparan sulfate and chondroitin sulfate. Inherited GUSB deficiency causes mucopolysaccharidosis type VII (Sly syndrome). GUSB is studied in lysosomal storage disorders and is also used as a reference gene in expression studies.
| Target | Beta-glucuronidase (gene GUSB; UniProt P08236, human) |
|---|---|
| Clone | 25G35 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 74.7 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GUSB.
Reactivity Notes
The supplier lists reactivity with human. UniProt places beta-glucuronidase in the lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
