| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein-glutamine gamma-glutamyltransferase 2;2.3.2.13;Tissue transglutaminase;Transglutaminase C;TG (C);TGC;TGase C;Transglutaminase H;TGase H;Transglutaminase-2;TGase-2;TGM2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human GUSB |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-GUSB antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25G35; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-GUSB Rabbit Monoclonal Antibody catalog # M02234. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: GUSB (Protein-glutamine gamma-glutamyltransferase 2).
- Antibody format: Monoclonal; clone 25G35; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
GUSB (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Reported cellular localization context: Secreted, extracellular space, extracellular matrix . Tissue expression notes (as provided): Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. .
Research relevance and current trends
- Research context keywords from the source record include: Amino Acid Metabolism,Amino Acids,Apoptosis,Associated Proteins,Autoimmune,Calcium Binding Proteins,Calcium Signaling,Cancer,Cell Biology,Immune System Diseases,Immunology,Intracellular,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction,Signaling Pathway.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate GUSB antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect GUSB expression by Western blot in cell or tissue lysates, Detect GUSB in FFPE tissue sections by immunohistochemistry, Localize GUSB by immunofluorescence/immunocytochemistry in cultured cells, Quantify GUSB-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 75 kDa; calculated MW: 77329 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 75 kDa
- Cellular localization (provided): Secreted, extracellular space, extracellular matrix .
- Tissue details (provided): Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
