| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cadherin-13; Heart cadherin; H-cadherin; P105; Truncated cadherin; T-cad; T-cadherin; CDH13; CDHH |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human H Cadherin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20C70 is a rabbit monoclonal antibody that recognizes cadherin-13 (gene CDH13) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 78.3 kDa; the supplier reports observed bands at 105, 130 kDa.
Cadherin-13 (CDH13), also called T-cadherin or H-cadherin, is an atypical cadherin that lacks transmembrane and cytoplasmic domains and is attached to the membrane by a GPI anchor. It acts as a receptor for adiponectin and LDL and regulates neural and vascular cell growth, making it relevant to cardiovascular and neurodevelopmental research.
| Target | Cadherin-13 (gene CDH13; UniProt P55290, human) |
|---|---|
| Clone | 20C70 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 78.3 kDa |
| Observed MW | 105, 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal heart.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human cervical cancer tissue, human squamous cells carcinoma tissue, mouse skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human H Cadherin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cadherin-13 in the cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
