| Field | Specification |
|---|---|
| Target | |
| Alternative names | Testis-specific H1 histone; Haploid germ cell-specific nuclear protein 1; Histone H1.7; Histone H1t2; H1-7; H1FNT; HANP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human H1-7 recombinant protein (Position: H35-R130). Human H1-7 shares 61.3% and 64.9 amino acid (aa) sequence identity with mouse and rat H1-7, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes testis-specific H1 histone (gene H1-7) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
Testis-specific H1 histone (H1-7, also called HANP1 or H1T2) is a linker histone expressed in developing male germ cells. It is needed during spermiogenesis for chromatin condensation, nuclear reshaping and the replacement of histones by protamines, and its loss impairs male fertility in mice. It is studied in sperm chromatin remodeling and male infertility.
| Target | Testis-specific H1 histone (gene H1-7; UniProt Q75WM6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human H1-7 fragment (His35–Arg130), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places testis-specific H1 histone in the nucleus and chromosome. Tissue expression noted by UniProt (human): Testis-specific.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
